rabbit polyclonal  (Proteintech)

Journal: Veterinary Research

Article Title: Outer membrane vesicles secreted by avian pathogenic Escherichia coli promote its survival within macrophages and systemic infection by inducing endoplasmic reticulum stress-mediated autophagy flux blockade

doi: 10.1186/s13567-025-01679-6

Figure Lengend Snippet: APEC OMVs induce incomplete autophagy in HD11 cells . A HD11 cells were treated with OMVs (100 µg/mL) for 6 h, and typical double-membrane autophagosomes were observed by transmission electron microscopy. Scale bar,500 nm. B , C HD11 cells were treated with OMVs (100 µg/mL) for 0–9 h, western blot analysis was performed to detect the expression of LC3-II, p62, and Beclin1 proteins in cell lysates, and gray value analysis was performed using ImageJ software ( C ) ( n = 3). D Immunofluorescence was used to detect the effect of OMVs (100 µg/mL) on the colocalization of LC3 and the lysosomal marker protein LAMP1 in HD11 cells. The data are representative of three independent experiments. Scale bar, 5 μm. Colocalization scatter plots were generated using ImageJ software and GraphPad Prism 8. n represents three biological replicates, with each data point corresponding to an independent culture system. Bar charts display mean ± standard error of the mean (SEM), analyzed by Student’s -test (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Rabbit anti-Beclin1 polyclonal antibody , Bioss, Beijing, China , 1:2000.

Techniques: Membrane, Transmission Assay, Electron Microscopy, Western Blot, Expressing, Software, Immunofluorescence, Marker, Generated